Cells of strain gss09t were anaerobic, gramstainpositive, motile, endosporeforming and rodshaped. Theflask was connected to an absorption train of two test tubes, eachcontaining. A summary of the diversity and biochemistry of bacteria that carry out a dissimilatory sulfate reduction has been given in chapter 24. Crude preparations of desulfotomaculum nigrificans were found to reduce bisulfite to trithionate, thiosulfate, and sulfide. A novel dissimilatory feiiireducing bacterium, designated strain gss09t, was isolated from a compost sample by using a solid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. We thrive on creating innovative biological reference materials that make microbiologists lives easier. This study focused on the physiological, chemotaxonomic, and genotypic characteristics of two thermophilic sporeforming sulfatereducing bacterial strains.
Get a printable copy pdf file of the complete article 243k, or click on a page image below to browse page by page. Desulfotomaculum nigrificans strain dsm 14880 vkm b2319 co. To atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. Sulfatereducing bacteria that form heatresistant endospores are classified in one genus, desulfotomaculum campbell and postgate, 1965. Desulfotomaculum ruminis desulfotomaculum nigrificans desulfotomaculum australicum desulfotomaculum thermobenzoicum desulfotomaculum geothermicum desulfotomaculum sp. Comparative 16s rdna sequence analysis revealed a relationship of the new isolate to desulfotomaculum ruminis and d. Fractionnegative d121 degrees cvalues obtained in modified soy formula were 25. Cells of this strain, designated rl50jiiit, were rodshaped and motile. You can merge pdfs or a mix of pdf documents and other files. The isolate is described as a new species, desulfotomaculum aeronauticum. Uvadare digital academic repository genome analyses of. Full text get a printable copy pdf file of the complete article 292k, or click on a page image below to browse page by page. A type of sulfatereducing bacteria, desulfotomaculum can cause food spoilage in poorly processed canned foods.
Links to pubmed are also available for selected references. This may include existing names the approved lists of bacterial names as well as new names and new combinations. Pereira 4, gerard muyzer 5, jan kuever 6, alexander v. Merge pdf files combine pdfs in the order you want with the easiest pdf merger available. Full text full text is available as a scanned copy of the original print version. Biocorrosion of dental alloys due to desulfotomaculum.
If your open pdf has more than a single page, select a thumbnail in the lefthand side where you want to insert another pdf file. Desulfotomaculum nigrificans formerly clostridium nigrificans is a thermophilic, anaerobic sporeformer that uses sulfates, sulfites, and reducible sulfur compounds as terminal electron acceptors, resulting in h2sproduction. A moderately thermophilic, grampositive, endosporeforming, sulfatereducing bacterium was isolated from a fluidizedbed reactor treating acidic water containing metal and sulfate. Heat resistance of nigrificans spores in soyprotein infant. Thiosulfate reductase isolated from desulfotomaculum. An increase in incubation period there is a dramatic increase in weight loss of polythene bag. By colorimetric methods of analysis, the products of bisulfite reduction by this enzyme were determined to be trithionate, thiosulfate, and sulfide. Desulfotomaculum is a genus of grampositive, obligately anaerobic soil bacteria. The strain, designated re35e1t, was rodshaped and motile. Drilling, coring, and sampling subsurface environments, in manual of. Doelle, in bacterial metabolism second edition, 1975. Here, we present its draft genome in which we found genes related with the anaerobic respiration of sulfur compounds similar to those present in the copahue environment.
With innerand outerlabeled 35sthiosulfate, the enzyme reduced only the outer sulfur atom to hydrogen sulfide. Pdf genome analyses of the carboxydotrophic sulfatereducers. Desulfotomaculum adalah sebuah genus bakteri grampositif yang memiliki sifat pereduksi sulfat, artinya, menggunakan sulfat untuk oksidasi, alihalih oksigen. Phylogenetic analysis of desulfotomaculum thermobenzoicum.
With innerand outerlabeled 35 sthiosulfate, the enzyme reduced only the outer sulfur atom to hydrogen sulfide. The type species of the genus is desulfotomaculum nigrificans approved lists 1980 emend. Click the button below to download a pdf of our latest catalog. Desulfotomaculum nigrificans werkman and weaver 1927 campbell and postgate 1965, 361 al clostridium nigrificans werkman and weaver 1927, 63. Biodegradation of polythene bag using bacteria isolated. This comparison documents similarities as well as large differences between the. Desulfotomaculum nigrificans are moderately thermophilic members of the. Phylogenetic analysis of the genus desulfotomaculum. The bisulfite reductase p582 from desulfotomaculum nigrificans was purified to homogeneity as judged by polyacrylamide gel electrophoresis. To change the order of your pdfs, drag and drop the files as you want. The 16s rrna gene of the thermophilic sulfatereducing bacterium desulfotomaculum thermobenzoicum was amplified by polymerase chain reaction using two eubacterial consensus oligodeoxynucleotide primers flanking the majority of the 16s rrna gene, cloned, and sequenced. Rhodanese activity, partially purified from desulfotomaculum nigrificans, was shown to reside in a protein fraction separate from thiosulfate reductase.
Desulfotomaculum thermocisternum desulfotomaculum aeronauticum desulfotomaculum halophilum desulfotomaculum kuznetsovii. The genus desulfotomaculum is a heterogeneous group of sporeforming sulfatereducing bacteria. Genome analyses of the carboxydotrophic sulfatereducers desulfotomaculum nigrificans and desulfotomaculum carboxydivorans and reclassification of desulfotomaculum caboxydivorans as a later synonym of desulfotomaculum nigrificans. In this sense the code is also dealing indirectly with taxonomic opinions. However, as with most codes of nomenclature there are no mechanisms for formally recording taxonomic opinions.
Pada 2005, spesies baru desulfotomaculum ditemukan di sebuah bekas pertambangan emas di mponeng, dekat johannesburg, afrika selatan, dan ditemukan dapat melangsungkan hidup tanpa tergantung. A thiosulfate reductase from desulfotomaculum nigrificans has been partially purified by ammonium sulfate fractionation, diethylaminoethylcellulose chromatography, and sucrose density gradient centrifugation. In the menu at the top, select view make sure thumbnails is checked in the dropdown menu. Isolation of tocopherol from desulfotomaculum nigrificans. Review of desulfotomaculum species and proposal of the. How to combine pdf files into one document lifewire. Full text get a printable copy pdf file of the complete article 645k, or click on a. In thiosulfate reductase isolated from desulfotomaculum. This study focused on the physiological, chemotaxonomic, and genotypic characteristics of two thermophilic sporeforming sulfatereducing bacterial strains, 435 t and 781, of which the former has previously been assigned to the subspecies desulfotomaculum nigrificans subsp. Desulfotomaculum nigrificans identified in a previous study was used in this work liu et al.
Draft genome sequence of the sulfatereducing bacterium. Additional information there are other files related to this item in huscap. The first isolate of this genus, a moderate thermophile, was originally described as clostridium nigrificans. Both strains reduced sulfate with the resulting production of h 2 s. These include anaerobic bacteria that reduce sulfates srb, sulfatereducing bacteria 88,94,95, as desulfovibrio vulgaris, desulfuricans, fairfieldensis, gigas or desulfotomaculum nigrificans. How to merge pdfs and combine pdf files adobe acrobat dc. The culture medium for the srb seed culture had a composition of g l. Desulfotomaculum copahuensis strain cindefi1 is a novel sporeforming sulfatereducing bacterium isolated from the copahue volcano area, argentina. Evidence for the misclassification of desulfotomaculum guttoideum and description of desulfotomaculum orientis as desulfosporosinus orientis. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. The bacteriological code deals with the nomenclature of prokaryotes. Desulfotomaculum nigrificans werkman and weaver campbell. Whether its customized environmental isolate controls, process controls, certified reference materials, proficiency samples, or something thats never been done before were up to the challenge.
Their presence can be identified by the release of hydrogen sulfide gas with its rotten egg smell when the can is first opened. Observations on the bisulfite reductase p582 isolated. Frontiers microbiologically influenced corrosion of. The results of phylogenetic analysis demonstrated that the genus desulfotomaculum already has lost the clustering monophyly and was segregated into some. The bisulfite reductase of this organism was partially purified and observed to reduce bisulfite to trithionate as the major product and with thiosulfate and sulfide as minor products. Pdf description of desulfotomaculum nigrificans subsp. Heat resistance of desulfotomaculum nigrificans spores in. Click, drag, and drop to reorder files or press delete to remove any content you dont want.
Desulfotomaculum species are common in anoxic freshwater, brackish or marine sediments. Genome analyses of the carboxydotrophic sulfatereducers. Pdf genome analyses of the carboxydotrophic sulfate. The studies of bacterial proliferation and an analysis of the chemical composition of sample surfaces were conducted. Pdf phylogenetic analysis of the genus desulfotomaculum. Although the majority of the sulfatereducing bacteria are unable to utilize glucose, desulfotomaculum nigrificans 37, which is a thermophilic member of the sulfatereducing bacteria 36, can be adapted to utilize glucose as an energy source 5. Anaerobic bacteria are found in the mouth due to the presence of socalled transitional flora. They are members of the phylum firmicutes, class clostridia, order clostridiales and family peptococcaceae kuever and rainey, 2009. Desulfotomaculum are usually found living in underground water, inside sediment or rock, or within feces of animals mainly pigs where little oxygen has reached.
Desulfotomaculum nigrificans and desulfotomaculum carboxydivorans and reclassification of desulfotomaculum caboxydivorans as a later synonym of desulfotomaculum nigrificans michael visser 1, sofiya n. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. Observations on the rhodanese activity of desulfotomaculum. A thermophilic, grampositive, endosporeforming, sulfatereducing bacterium was isolated from an underground mine in a geothermally active area in japan. Notification of changes in taxonomic opinion previously. Desulfotomaculum nigrificans in degradation of polythene bag at 30 days. Rearrange individual pages or entire files in the desired order. Desulfotomaculum nigrificans dsm574t x62176 thermosinus carboxydivorans dsm 14886t nr117169. Get a printable copy pdf file of the complete article 1. Desulfotomaculum carboxydivorans strain dsm 14880 vkm b2319 co1 srb is moderately thermophilic, anaerobic.
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